Research Specialist B/C

Job Description

Job Responsibilities Research Specialist B

• Conduct in vitro immunological functional assays, including CAR T cell manufacturing, flow cytometry, and ELISA, following established protocols; troubleshoot experimental issues as they arise

• Conduct in vivo mouse studies, executing established protocols under the direction of the PI

• Prepare tumor cells and engineered immune therapies for in vivo experiments, documenting their characteristics and performing assays to assess functional performance

• Monitor and collect datasets associated with animal studies, including health monitoring and weights, tumor growth (caliper, bioimaging), bleeds, and necropsies. Perform IV, IT, IP, and SC injections of tumor cells and therapeutics.

• Prepare tissues at necropsy by fixing and isolating cells for functional and flow cytometric analysis, IHC, and spatial transcriptomic studies.

• Document experimental data, perform data analysis, and contribute to the preparation of presentations and research publications under the guidance of the Principal Investigator.

• Participate in lab management and support lab set-up and operations, including ordering, organizing, and maintaining reagents, equipment, and protocols

• Characterize transgenic mice and maintain colony.

• Train and provide guidance to other lab members as needed.

Job Responsibilities Research Specialist C

• Independently design, troubleshoot, and perform in vitro immunological functional assays, including CAR T cell manufacturing, flow cytometry, and ELISA; contribute to development of new methods using state-of-the-art techniques.

• Independently design, troubleshoot, and execute in vivo mouse studies using established protocols, and contribute to development of new protocols under the direction of the lab supervisor

• Modify and prepare tumor cells and engineered immune therapies for in vivo mouse experiments, documenting their characteristics and performing assays defining functional performance.

• Monitor and collect datasets associated with animal studies, including health monitoring and weights, tumor growth (caliper, bioimaging), bleeds, and necropsies. Perform IV, IT, IP, and SC injections of tumor cells and therapeutics

• Prepare tissues at necropsy by fixing and isolating cells for functional and flow cytometric analysis, IHC, and spatial transcriptomic studies.

• Document experimental data, perform data analysis, generate and organize the data needed to support grant applications and IND-enabling studies, and write sections of manuscripts and progress reports describing research results.

• Provide scientific and technical guidance and mentorship to other lab members and trainees, contributing to research team performance.

• Manage day-to-day research bench operations, including maintaining general lab supplies and reagent inventory, equipment upkeep, and protocol organization

• Characterize transgenic mice and maintain colony.

Position is funded for one year with the possibility of being extended.

Qualifications

Required:

• Research Specialist B:

• Bachelor's degree in Immunology, Cell Biology, Biomedical Sciences, or a related field, with 1–2 years of relevant research experience, or an equivalent combination of education and experience

• Hands-on experience with in vitro immunological assays, including flow cytometry, ELISA, and cell culture

• Exposure to in vivo mouse models, including tumor inoculation, therapeutic administration, or monitoring

• Strong organizational and documentation skills

• Research Specialist C:

• Bachelor's degree in Immunology, Cell Biology, Biomedical Sciences, or a related field, with 3–5 years of relevant research experience, or an equivalent combination of education and experience

• Hands-on experience with in vitro immunological assays, including flow cytometry, ELISA, cell culture, and co-culture killing assays

• Experience with in vivo mouse models, including tumor inoculation, therapeutic administration, and monitoring

• Experience with immunotherapy

• Strong organizational and documentation skills

Preferred:

• Experience with CAR T cells or cell-based immunotherapies

• Experience with co-culture killing assays

• Experience with tissue processing techniques, including IHC and spatial transcriptomics

• Experience with bioimaging for tumor growth assessment

• Familiarity with characterizing transgenic mice or maintaining mouse colonies, including immunodeficient strains (e.g., NSG)

• Prior contribution to research presentations or publications

• Familiarity with bioinformatics approaches for spatial transcriptomics or other high-dimensional datasets

• Familiarity with microbiology

• Job Location - City, State

• Philadelphia, Pennsylvania

• Department / School

• Perelman School of Medicine

• Pay Range

• $50,875.00 - $67,794.79 Annual Rate

• Salary offers