Research Opportunities - McDonnell Genome Institute

Location

ST. LOUIS, MO 63108

Scheduled Hours 40

Position Summary We are seeking a highly motivated scientist to join our functional genomics team at the Goldfarb Center for Neuronal Resiliency at the McDonnell Genome Institute, WashU Medicine. The Goldfarb Center for Neuronal Resiliency was established to identify new therapeutic targets and treatments for neurodegenerative diseases, particularly ALS and Parkinson’s disease.

Central to this effort are CRISPR screens that identify genes that make neurons more vulnerable or resilient to stress. These screens will use human induced pluripotent stem cell (iPSC)-derived neuronal models of neurodegenerative diseases. Work in the lab also integrates CRISPR perturbations with phenotypic assays and molecular readouts using flow cytometry, high content imaging, next-generation sequencing, and single-cell, spatial, and in situ profiling.

About The Goldfarb Center for Neuronal Resiliency

Led by Drs. Jeff Milbrandt and Rob Mitra, The Goldfarb Center for Neuronal Resiliency is a highly collaborative and interdisciplinary research environment integrating functional genomics, stem cell engineering, neuroscience, high throughput imaging, next-generational sequencing, artificial intelligence, and drug discovery. Our work is inspired by the observation that neurodegeneration is strikingly selective for specific neuronal populations. For example, in ALS, motor neurons degenerate while neighboring interneurons remain intact. Even within motor neurons, some subtypes are highly vulnerable, while others are resilient. We believe these differences in resiliency provide clues to new therapeutic strategies that have yet to be harnessed for drug development. Specifically, our long-term goal is to discover therapeutics that activate resiliency factors or inhibit vulnerability factors in diseased neurons in order to restore them to heath and reverse the progression of these debilitating diseases.

Job Description

Primary Duties & Responsibilities:

For more about this project, visit https://genome.wustl.edu/the-resiliency-project-advancing-neurodegenerative-disease-research-at-mgi/.

Working Conditions:

• Because some cell culture, differentiation, and time-sensitive experimental workflows require procedures on specific days, the successful candidate must be able to accommodate occasional weekend work when required by experimental schedules.
• This position works in a laboratory environment with potential exposure to biological and chemical hazards.
• The individual must be physically able to wear protective equipment.

Salary Range: Base pay is commensurate with experience.

The above statements are intended to describe the general nature and level of work performed by people assigned to this classification. They are not intended to be construed as an exhaustive list of all job duties performed by the personnel so classified. Management reserves the right to revise or amend duties at any time.

Required Qualifications

Education

• Bachelor’s degree or combination of education and/or experience may substitute for minimum education. Certifications/Professional Licenses:
• No specific certification/professional license is required for this position. Work Experience:
• No specific work experience is required for this position.

Skills

• Not Applicable Driver's License:

• A driver's license is not required for this position.

• More About This Job

Preferred Qualifications:

• Candidates with a bachelor's, master's degree, or PhD will be considered.
• Substantial research experience in molecular biology, cell biology, genetics, genomics, neuroscience, bioengineering, or a related field.
• Strong hands-on experience in mammalian cell culture and molecular biology.
• Strong organizational skills and ability to manage multiple experiments and priorities.
• Strong written and verbal communication skills.

Preferred Experience: Experience in one or more of the following areas would be highly desirable:

• CRISPR/Cas genome editing, CRISPR screening, or functional genomics.

• Human iPSC culture and differentiation, particularly neuronal differentiation.

• Lentiviral vector production and transduction.

• Flow cytometry and fluorescence-activated cell sorting (FACS).

• Reporter assay development and engineered stable cell lines.

• qRT-PCR and Western blotting.

• DNA/RNA purification, PCR, cloning, and other molecular biology techniques.

• Next-generation sequencing and sequencing-library preparation.

• Experience with every technique listed above is not required. Candidates with a strong foundation in mammalian cell and molecular biology, demonstrated scientific independence, and the ability to rapidly learn new technologies are encouraged to apply.

• Final appointment and title will be commensurate with the candidate's qualifications and experience.

• Preferred Qualifications

Education

• No additional education unless stated elsewhere in the job posting. Certifications/Professional Licenses:
• No additional certification/professional licenses unless stated elsewhere in the job posting. Work Experience:
• No additional work experience unless stated elsewhere in the job posting.

Skills

• Communication, Laboratory Research, Organizing, Recordkeeping, Working Independently

• Grade

• 00

• Salary Range

• Base pay is commensurate with experience.

• The salary range reflects base salaries paid for positions in a given job grade across the University. Individual rates within the range will be determined by factors including one's qualifications and performance, equity with others in the department, market rates for positions within the same grade and department budget.

• Questions

• For frequently asked questions about the application process, please refer to our External Applicant FAQ.